TPHRL has three separate systems for tobacco smoke consisting of 8 whole body exposure chambers. Each system has a smoke generating machine and either 2 or 3 Hinners-type chambers connected to a carbon monoxide analyzer. In addition, a diffusion flame system is available to serve as a surrogate to diesel engine (combustion) exposure.

TPHRL has a nose-only exposure system for aerosolized delivery of gases, vapors, and particles to multiple ports. This system has been successfully used for the study of electronic-cigarettes and is adaptable for other types of nose-only exposures.

A basic biochemistry lab is available with multiple fume hoods and equipment and bench space for analyses to be done. Room is dedicated to tissue processing, slicing, and embedding as well.

A surgical suite is located in the facility for small animal minor surgical procedures, such as oropharyngeal aspiration, intranasal administration of compounds, and pulmonary testing.